Advanced Light Microscopy and Spectroscopy Laboratory
Equipment
Leica Confocal SP8-SMD
From live cell to quantitative imaging, from super-sensitivity to super-resolution, from multiphoton to light sheet imaging – with the Leica TCS SP8 Confocal platform you can always expand your possibilities. We build on a modular concept: Tailor your microscope to your current needs and upgrade additional functionalities at any time.
Single molecule detection (SMD) and analysis is an elegant way to examine dynamics and interactions inside cellular systems. The new Leica TCS SMD Series perfectly integrates the confocal microscope Leica TCS SP8 and the SMDspecific software and hardware from PicoQuant into one system.
The well proven components constitute a flexible platform for a plethora of time resolved techniques including FCS , FCCS, FLIM and FLCS. The global system control and its elegant interface open the door to a multitude of biophysical techniques which provide quantitative parameters for model building.
- Leica Inverted DMI8 microscope stand.
- 2 PMTs, 3 HyD (hybrid detectors).
- CO2/temperature controlled Tokai Hit system for imaging of live cells.
- Single photon lasers (attenuated using acousto-optical tuning filters (AOTFs)).
- argon laser: excitation lines at 458, 476, 488, 496 and 514 nm (blue-cyan).
- White light laser for excitation at any wavelength in the visible range.
- 405 nm violet (near UV) laser for near UV excitation.
- Transmitted light detection.
- Single molecule detection system (SMD) using photon counting.
- Fluorescence Lifetime imaging (FLIM).
- Fluorescence Correlation Spectroscopy (FCS).
- Huygens Professional software for image processing.