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Advanced Light Microscopy and Spectroscopy Laboratory

Providing a unique collection of leading-edge optical microscopes, small-animal imaging devices, and fluorescent probes

Advanced Light Microscopy and Spectroscopy Laboratory

Equipment

Leica TCS-SP5 AOBS Confocal Microscope

Main Use: Standard and fast scanning confocal microscopy through the visible spectrum

The Leica TCS SP5 Confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. The system provides the full range of scan speeds at the highest resolution.

With its proven, high-efficiency SP detection (five channels simultaneously) and AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed.

  • Leica Inverted DMI6000 microscope stand.
  • DIC, 6 PMTs.
  • CO2/temperature controlled enclosure for time-lapse imaging of live cells.
  • Single photon lasers (attenuated using acousto-optical tuning filters (AOTFs)).
  • Argon laser:excitation lines at 458, 476, 488, 496 and 514 nm (blue-cyan).
  • 3 helium-neon lasers: excitation lines at 543 (green), 594 (orange) and 633 (far red) nm.
  • 405 nm violet (near UV) laser for near UV excitation.
  • Acousto-Optical Beam Splitter (AOBS).