New Single Molecule Detection Platform at CNSI


Meghan Steele Horan | October 7, 2016

The Leica TSC SP8 Single Molecule Detection Microscope is now available for use in the California NanoSystems Institute. (Image credit CNSI)

The California NanoSystems Institute (CNSI) at UCLA is pleased to announce the recent acquisition of the newest Single Molecule Detection confocal microscope (TCS SP8 SMD) from Leica Microsystems, a leading developer and manufacturer of optical microscopes and imaging systems, to support its research efforts in nanoscience and nanotechnology.

“CNSI and the entire UCLA research community will greatly benefit from this new state-of-the-art confocal microscope. This equipment will enhance our ability to image at the nanoscale” says Dr. Laurent A. Bentolila, Scientific Director of the Advanced Light Microscopy/Spectroscopy Technology Center at CNSI.

The Advanced Light Microscopy/Spectroscopy laboratory is one of six shared resource technology centers at CNSI. The lab focuses on optical imaging and advanced image-analysis techniques for the study of macromolecules, cellular dynamics and nanoscale characterization of biomaterials.
“Techniques such as FRAP, FLIP, FRET, FCS and FLIM have transformed microscopes into real-time biometric readers where molecular interactions and intracellular dynamics can be studied inside cellular compartments” says Bentolila.

The Leica TSC SP8 SMD includes an extensive image analysis wizard for 3D and 2D analysis of multi-dimensional data sets. (Image credit CNSI|ALMS)

Research that will benefit from the new Leica TCS SP8 SMD microscope includes dynamic studies in live cells, biochemical analysis, multispectral/multicolor analysis, low-light imaging, single molecule detection and super-resolution imaging down to ~140 nm.

The Leica TCS SP8 SMD microscope will augment and expand a wide array of optical imaging systems already at the ALMS lab. These instruments are offered on a shared basis and are open to all classes of users academic and industrial. Training is provided for all instruments after which users are given 24 hours a day, 7 days a week access. The ALMS Technology Center has served as a catalyst for multidisciplinary collaborations across campus in the fields of health, medicine, energy and the environment.

Interested users may contact Dr. Laurent A. Bentolila, Scientific Director at lbento@chem.ucla.edu for further inquiries, consultation and proof-of-concept. Registration and training can be scheduled at alms@cnsi.ucla.edu.

For more information about the Advanced Light Microscopy and Spectroscopy Laboratory, please visit the ALMS website